A drop of methylene blue was placed in each set-up with an initial diameter of 10mm the diffusion rates of the methylene on the agar plate at a temperature of 0c is 0066, at 15c is 0126, at 25c is 032, at 40c is 0554, at 60c is 166. Use cubes of agar to investigate how size impacts diffusion choose one ph indictor to work with (either bromothymol blue or phenolphthalein) and add a few drops of it to the agar solution while this snack investigates how the size of an agar cube impacts diffusion, the shape of each cube. Eosin methylene blue agar, levine is a slightly selective and differential plating medium for the isolation of gram-negative enteric bacteria principles of the procedure the eosin y and methylene blue dyes in levine emb agar render the medium slightly selective in. Rate of diffusion of mythelin blue in agar jelly essay aim to measure how different concentrations of mythelin blue affects the rate of diffusion through agar jelly hypothesis the diffusion of mythelin blue is directly proportional to its concentration, hence as the concentration.
Powerpoint slideshow about 'diffusion rate chemical diffusion in agar' - aaron diffusion of dye in agar in agar, what size of molecules move the fastest which dye, the methylene blue or the potassium permanganate, diffused at the. A cubes of agar jelly placed into solutions of methylene blue or potassium permanganate will absorb the pigment by diffusion the cubes are left in the pigmented solution for different measured periods of time and are then sliced open the distance between the.
I will then calculate the rate of diffusion from this the reaction throughout the agar segments will depend on the collisions between the analysis statement about the graph the graph shows that the rate of diffusion has increased with the concentration of the. Diffusion is the movement of particles from an area where their concentration is high, to an area where thier concentration is low at higher temperatures you are giving the hydrochloric acid molecules more kinetic energy, so they will diffuse into the agar quicker, and make them clearer- i assume they had.
The diffusion process of methylene blue aqueous solutions in agar samples was ana- lyzed by the pa technique ing the process of diffusion of the methylene blue into the agar this last assumption is valid for low concentrations of methylene blue only it is. This can be helpful in understanding how diffusion in water occurs in different physical conditions materials 150 ml of hot tap, cold tap blue dye data dye methylene blue methylene blue methylene blue methylene blue methylene blue methylene blue. To the rate of diffusion using methylene blue and potassium permanganate through agar gel test from anti essays, your also, to determine which of the two substances being tested diffused fast six agar plates that contained an agar gels were used and. Eosin methylene blue (or emb) agar is a selective & differential medium it is selective because it encourages some bacteria to grow while inhibiting others eosin y and methylene blue generally inhibits gram positive bacteria from growing (a few will grow.
Agar jelly is a type of jelly using the seaweed gelatin know as agar agar as a thickener usually it is used in europe because it does not appeal to the the rate of diffusion depends on the surface area, an increase in the temperature and pressure will result in the increased rate of diffusion in addition. Methylene blue is used as dye to identify bacteria and nucleic acids the dye will have the deepest shade of blue when in contact with acids as indicators and oxidizing agents, the substances stated above must prove that their diffusion must be fast in order to do their. Diffusion-of-methylene-blue-in-phantoms-of-agar-using-optical-absorption-techniques .
Compute the rate of diffusion of the potassium permanganate molecules in millimeters per minute as well as the rate of methylene blue prepare the lab report together with your introductions, hypothesis, discussions, results, conclussions, and then the references. The diffusion rates of the methylene on the agar plate at a temperature of 0c is 0066, at 15c is 0126, at 25c is 032, at 40c is 0554, at 60c is 166 high concentration gradient leads to greater rate of diffusion blue & yellow) tap water ice stopwatch to test the. Eosin methylene blue (emb) agar is both selective and differential it contains the dyes eosin and methylene blue, which inhibit the growth of gram-positive bacteria and therefore select for gram-negative bacteria it also contains the carbohydrate lactose, which allows. Eosin methylene blue (emb, also known as levine's formulation) is a selective stain for gram-negative bacteria emb contains dyes that are toxic to gram-positive bacteria.
Eosin methylene blue agar micro lab exam 2 what type of agar is eosin methylene blue (emb) agar is a complex, selective, and differential medium. Aim to measure how different concentrations of mythelin blue affects the rate of diffusion through agar jelly controlled variables time time was kept constant while testing the diffusion spread of mythelin blue with each concentration. Eosin methylene blue agar (emb) is a selective and differential medium used to isolate fecal coliforms eosin y and methylene blue are ph indicator dyes which combine to form a dark purple precipitate at low ph they also serve to inhibit the growth of most gram.